Broilers and Turval probiotic effects.

Trial 19: The results of this trial demonstrate that Turval B0399 is able to exercise its probiotic action with evident auxinic effects.

B. Braikovich DVM –Padova Italy; G. Menis-Artegna (UD) Italy

The subjects naturally did not yet have an intestinaI bacterial microflora which was already developed and bio regulated. They were raised in excellent environmental and hygienic conditions, and did not undergo any particular forms of stress.

Summary

TURVAL B0399, in the ration respectively of 1kg per 100 kg of formulated Starter -type feed, 200 g per 100 kg of Grower – type feed, 50g per 100 kg of Finishing-type feed, produced the following interesting results (treated group vs. control): an increase in average live weight per head (+7.75 %); a lower conversion index (-5.75 %); lower mortality (-16.00 %).

Methodology of the trial
The trials were carried out at 3 breeding farms affiliated with a well-known feed factory of the area (Blasizza, Inc.). 9000 young subjects (broilers) were used in total, 4500 of which were treated with TURVAL B0399 from birth to time of slaughter and 4500 used as the control group.

The distribution of the groups was organized as follows:

Breeding farm 1: 2000 control and 2000 treated

Breeding farm 2: 1500 control and 1500 treated

Breeding farm 3: 1000 control and 1000 treated

Limited yet homogeneous areas of the farms were used and which had the same climatic and environmental conditions for both the treated fowl and the fowl in the control groups.

The chickens from 0 to 3 weeks old were given a starter feed.

From the 4^th to 7^th week they were given a grower feed.

The last 10 days, a finishing feed.

Slaughtering took place the 50th or 51st day.

In every farm or section of the same, the same quantity of feed was administered to both the treated and control groups.

Materials
All feeds were produced by the company Blasizza Mills of Farra d’ Isonzo (GO), authorization n° MG 749/RA/1016, April 12, 1991.

S: Starter type feed.

Components: corn flour, cornflakes, wheat bran, flour of extract of soy, wheat starch, powdered whey, barley flour, oats, calcium carbonate, molasses, monocalcic phosphate, sodium chloride, lysine Hcl, DL – methionine, zinc oxide, aroma.

G: Grower type feed.

F: Finishing type feed.

TURVAL B0399:probiotic additive.

Components of additive TURVAL B0399: typified active lactic yeast Kluyveromyces F. B0399, wheat bran pre-treated and sterilized, whey powder, lactose, hydrolysed casein.

Lactic yeast Kluyveromyces B0399 > 5 x 10^6 CFU/gr

Results of the trial

The charts below show indicate the results:

Breeding Farm 1

Breeding Farm 1	N° control	N° treated	Variation %
Beginning	2125	2024
+ 51 days	2057	1957
Mortality	3.2%	2.4%	- 25 %

Breeding Farm 1	N° control	N° treated	Variation %
+ 51 days	2057	1957
Total live weight (kg)	4.400.	4.600
Live weight per head	2.130	2.350	+ 10.30 %
Average daily weight gain (in grams)	41.75	46.07	+ 10.30 %

Breeding Farm 1	N° control	N° treated	Variation %
Feed (kg)	9.700	9.700
Total live weight (kg)	4.400.	4.600
Conversion index	2.20	2.10	- 4.55 %

ALLEVAMENTO 2

Breeding Farm	N° control	N° treated	Variation %
Beginning	1510	1500
+ 50 days	1455	1464
Mortality	3,0%	2,4%	- 20 %

Breeding Farm	N° control	N° treated	Variation %
+ 50 days	1455	1464
Total live weight (kg)	3.050.	3.295
Live weight per head	2,090	2,250	+ 7,65 %
Average daily weight gain (in grams)	41,80	45,00	+ 7,65 %

Breeding Farm	N° control	N° treated	Variation %
Feed (kg)	6.000	6.000
Total live weight (kg)	3.050.	3.295
Conversion index	1,96	1,82	- 7,15 %

ALLEVAMENTO 3

Breeding Farm	N° control	N° treated	Variation %
Beginning	1026	1025
+ 51 days	998	1004
Mortality	2,80%	2,00%	- 28,5 %

Breeding Farm	N° control	N° treated	Variation %
+ 51 days	998	1004
Total live weight (kg)	2.225.	2.380
Live weight per head	2,230	2,370	+ 6,25 %
Average daily weight gain (in grams)	43,72	46,47	+ 6,25 %

Breeding Farm	N° control	N° treated	Variation %
Feed (kg)	4.500	4.500
Total live weight (kg)	2.225.	2.380
Conversion index	2,00	1.90	- 5,25

SUMMARIZATION CHART

	N° controllo	N° trattati	Total
Beginning	4.661	4.549	9.210
Tot. Subjects to slaughter	4.510	4.425	8.935
Mortality	3,25 %	2,72%	- 16,00 %

	N° controllo	N° trattati
Tot subjects to slaughter	4.510	4.425	8.935
Total live weight (kg)	9.675 3.050 a + 50 gg 6.625 a + 51gg	10.275 3.295 a + 50 gg 6.980 a + 51 gg	19.950
Live weight per head	2,090 (a + 50 gg) 2,170 (a + 51 gg) 2,140	2,250 (a + 50 gg) 2,350 (a + 51 gg) 2,310	+ 7,65 % + 8,03 % + 8,00 %
Average daily weight gain (in grams)	x 1.455 (50 gg) = 41,80 x 3.055 (51gg) = 42,54 42,30	x 1.464 (50gg) = 45,00 x 2.961 (51gg) = 45,07 45,71	+ 7,65 % + 8,30 % 8,05 %

	N° controllo	N° trattati
Feed (kg)	20.200	20.200	40.400
Total live weight (kg)	9.675	10.275	19.950
Conversion index	2,08	1.96	- 5,75

An increase in the live weight in the treated groups (2.350 kg as compared to 2.140 kg in the non-treated groups) of 170 grams which is equivalent to + 8%.

The weight increase determined a major daily average weight gain per head in the treated groups (45.71g compared to 42,30 in the non-treated groups)of 3.41 g which is equivalent to + 8,05%,

The weight gain was evident in the growing phase (after the 3rd week) and above all in the finishing phase (after the 6th week) when the treated groups also increased their ingestion.

From the moment that all groups (control and treated) were treated with the same quantity of feed, the increase of live weight in the treated subjects determined a major feed yield and a decrease of the conversion index equal to 5.75%

Since the groups were fed with the same quantity of feed it was not possible to determine the conversion index with precision, but the fact that an indirect reduction of the conversion index was observed implicates that it could have been substantially lower (- 20-25 %)

A lower mortality rate was also observed (- 16 %).

This value is significant considering that the mortality rate, even in the control groups, was maintained low, almost physiological

The purpose of the trial was to verify if the probiotic (TURVAL B0399) is able to create an auxinico effect (a major plastic yield) in poultry (broilers), raised according to the most modern techniques of breeding, diet and sanitary hygiene.

The subjects naturally did not yet have an intestinaI bacterial microflora which was already developed and bio regulated

They were raised in excellent environmental and hygienic conditions, and did not undergo any particular forms of stress.

The results of this trial demonstrate that Turval B0399 is able to exercise its probiotic action with evident euxinic effects.