Trial 27: Utilization of a probiotic product based on the lactic yeast Kluyveromyces marxianus fragilis B0399 (Turval B0399) in the diet of sleddogs (huskies).

Ararad Khatchikian – Huskies breeder and teacher of the Mushing international school of Tarvisio (Italy)

Object: trial No. 27

Trial of utilization of probiotics with a basis of typified lactic yeasts (Turval B0399) in the diet of sleddogs (huskies).

Ararad Khatchikian – Huskies breeder and teacher of the Mushing international school of Tarvisio (Italy)

Objective.

The objective of the trial was to observe the effects of the utilization of the new probiotics with a basis of typified lactic yeasts Kluyveromyces B0399 (Turval B0399) in the diet of sleddogs.

In particular, the digestive functions and general health of the dogs were observed.

Summary of results.

Individual charts were kept showing results relative to 7 dogs (3 male and 4 female) of ages between 13 months and 10 years.

Except for the dog Mille (charts 7 and 8), whose results were not evident due to a severe case of verminosis, the other 6 dogs had positive results with the addition of the probiotic Turval B0399; especially Alfa (charts 1 and 2), Yerghu (charts 9 and 10) and Yeregh (charts 11 and 12).

The product demonstrated to be most certainly valid and very interesting.

Materials

a- Base diet: Mixed: Croquettes + warm water (300 g + 2 liters of

water in 2 meals a day.)

b- Probiotic integration: TURVAL 25 DOG

Turval 25 Dog containes TURVAL B0399 (probiotic lactic yeast Kluyveromyces m/f B0399)

Manufacturing company: TURVAL Laboratories, Ltd.

Place of testing:

SCUOLA INTERNAZIONALE MUSHING Via Rutte Grande, 16 - C.P. 13633018 TARVISIO (UD) Italy Fax +39 0428 40566

Methods and results

See attached charts

CHART 1

GENERAL INFORMATION Subject treated: ALFA

Breed: HUSKY

Sex: FEMALE

Age: YEARLING - 13 MONTHS OLD

State of health at beginning of trial:

(Critical) Convalescence due to both a heavy hormonal treatment (which provoked a

vaginal infection) and antibiotic treatment. The dog was extremely weak and was

being administered phleboclyses of hysiological solution.

Duration of treatment: 4 weeks

Dosage used: 12 g in the morning meal

Incoveniences encountered during period of administration : none

CHART 2

DIARY CHART

Subject treated: ALFA

Observations during the course of administration period
General state of health	Beginning of administration	1st week	2nd week	3rd week	4th week	5th week
Excellent
Good				X	X
Fair			X
Critical	X	X
*Feces: consistency*
Liquid	X
Soft		X
Normal			X	X	X
Dry

*Feces: odor*
More acrid than normal	X
Normal			X	X	X
Less acrid than normal		X

*Coat*
Shiny			X	X	X
Dull	X	X
Crinkled

*Eyes*
Lively				X	X
Normale		X	X
Dull	X

*Weight of subject*	15 kg	16 kg	17 kg	19 kg	20 kg

Final comment :

At the beginning of the trial with TURVAL B0399 the dog was very weak and underwent fleboclyses of physiological solution. TURVAL B0399 definitely contributed in awakening its appetite and rebuilding the bacteric flora

CHART 3

GENERAL INFORMATION

Subject treated: ELIM

Breed: HUSKY

Sex: FEMALE

Age: ADULT - 7 YEARS OLD

State of health at beginning of trial: (fair) At the beginning of administration the dog had an intestinal parassitosis brought on by whipworm, which did not intefere with appetite and general behavior.

Duration of treatment: 4 weeks

Dosage used: 12 g in the morning meal

Inconveniences encountered during period of administration: none

CHART 4

DIARY CHART

Subject treated: ELIM

*Observations during the course of administration period*
General state of health	Beginning of administration	1st week	2nd week	3rd week	4th week	5th week
Excellent
Good		X	X	X	X
Fair	X
Critical

· *Feces: consistency*
Liquid	X
Soft		X	X	X	X
Normal
Dry

*Feces: odor*
More acrid than normal	X
Normal
Less acrid than normal		X	X	X	X

· *Coat*
Shiny		X	X	X	X
Dull	X
Crinkled

· *Eyes*
Lively
Normal	X	X	X	X	X
Dull


Weight of subject	20 kg	20 kg	21 kg	21 kg	22 kg

Final comment:

TURVAL B0399 improved the consistency of the feces though it did not resolve the problem of verminosis. After the fifth week the dog was dewormed and the feces returned to normal.

CHART 5

GENERAL INFORMATION

Subject treated: SCREAMER

Breed: HUSKY

Sex: FEMALE

Age: YEARLING - 20 MONTHS

Duration of treatment: 4 weeks

Dosage used:12 g in the morning meal

State of health at beginning of trial: (good) At the beginning of administration the dog was in heat and therefore had a lack of appetite.

Inconveniences encountered during period of administration: none

CHART 6

DIARY CHART

Subject treated: SCREAMER

*Observations during the course of administration period*
General state of health	Beginning of administration	1st week	2nd week	3rd week	4th week	5th week
Excellent
Good	X	X	X	X	X
Fair
Critical

· *Feces: consistency*
Liquid
Soft
Normal	X	X	X	X	X
Dry

*Feces: odor*
More acrid than normal
Normal	X	X	X	X	X
Less acrid than normal

· *Coat*
Shiny	X	X	X	X	X
Dull
Crinkled

· *Eyes*
Lively
Normal	X	X	X	X	X
Dull


Weight of subject	17 kg	17 kg	17 kg	18 kg	18 kg

Final comment:

TURVAL B0399 was administered to stimulate the poor appetite caused by the dog’s being in heat. The dog found it extremely palatable and even loved eating it in the bowl with no other additions.

CHART 6

GENERAL INFORMATION

Subject treated: MILLIE

Breed: HUSKY

Sex: FEMALE

Age: ADULT - 5 YEARS AND 11 MONTHS OLD

State of health at beginning of trial : (fair) At the beginning of administration the dog had no appetite, weakened by an intestinal parassitosis caused by whipworm.

Duration of treatment: 4 weeks

Dosage used : 12 g in the morning meal

In conveniences encountered during period of administration: none

CHART 7

DIARY CHART

Subject treated: MILLIE

*Observations during the course of administration period*
General state of health	Beginning of administration	1st week	2nd week	3rd week	4th week	5th week
Excellent
Good
Fair	X	X	X	X	X
Critical

· *Feces: consistency*
Liquid
Soft	X	X	X	X	X
Normal
Dry

*Feces: odor*
More acrid than normal	X	X	X	X	X
Normal
Less acrid than normale

· *Coat*
Shiny
Dull	X	X	X	X	X
Crinkled

· *Eyes*
Lively
Normal	X	X	X	X	X
Dull


Weight of subject	20 kg	20 kg	20 kg	20 kg	20 kg

Final comment :

The probiotic did not give results in this subject . The successive deworming after treatment with TURVAL solved the problem completely.

CHART 8

GENERAL INFORMATION Treated subject: YERGHU’

Breed: HUSKY

Sex: MALE

Età:ADULTO - 6 YEARS OLD

State of health at beginning of trial : (excellent) : The dog’s feces have always had a tendency of being soft and fetid

Duration of treatment : 4 weeks

Dosage used : 12 g in the morning meal

Inconveniences encountered during administration: none

CHART 9

DIARY CHART

Treated subject: YERGHU’

*Observations during the course of administration period*
General state of health	Beginning of administration	1st week	2nd week	3rd week	4th week	5th week
Excellent	X	X	X	X	X
Good
Fair
Critical

· *Feces: consistency*
Liquid
Soft	X
Normal		X	X	X	X
Dry

*Feces: odor*
More acrid than normal	X
Normal		X	X	X	X
Less acrid than normal

· *Coat*
Shiny		X	X	X	X
Dull	X
Crinkled

· *Eyes*
Lively
Normal	X	X	X	X	X
Dull


Weight of subject	22 kg	22 kg	23 kg	23 kg	24 kg

Final comment :

TURVALB0399 definitely changed the consistency and odor of the feces, contributing in improving the general state of health of the dog. The supplement proved to be quite palatable, even on its own.

CHART 10

GENERAL INFORMATION Subject treated: YERèGH

Breed: HUSKY

Sex: MALE

Age: ADULT - 6 YEARS OLD

State of health at beginning of trial: (excellent) The dog has always had a tendency towards soft and fetid feces

Duration of the treatment: 4 weeks

Dosage used :12 g in the morning meal

Inconveniences encountered during period of administration: none

CHART 11

DIARY CHART

Subject treated: YERèGH

*Observations during the course of administration period*
General state of health	Beginning of administration	1st week	2nd week	3rd week	4th week	5th week
Excellent	X	X	X	X	X
Good
Fair
Critical

· *Feces: consistency*
Liquid
Soft	X
Normal		X	X	X	X
Dry

*Feces: odor*
More acrid than normal	X
Normal		X	X	X	X
Less acrid than normal

· *Coat*
Shiny		X	X	X	X
Dull	X
Crinkled

· *Eyes*
Lively
Normal	X	X	X	X	X
Dull


Weight of subject	22 kg	22 kg	23 kg	23 kg	24 kg

Final comment:

TURVAL B0399 definitely changed the consistency and odor of the feces, contributing in improving the general state of health of the dog. The supplement was extremelyl palatable and was also enjoyed on its own without other additions

CHART 12

GENERAL INFORMATION Subject treated: SIMON

Breed: HUSKY

Sex: MALE

Age: ADULT - 10 YEARS AND SIX MONTHS OLD

State of health at beginning of trial: (good) At the beginning of administration the dog had a parassitosis caused by whipworm which did not interfere with appetite or general behavior.

Duration of treatment: 4 weeks

Dosage used: 12 g in the morning meal

Inconveniences encountered during administration : none

CHART 13

Diary Chart

Subject treated: SIMON

*Observations during the course of administration*
General state of health	Beginning of administration	1st week	2nd week	3rd week	4th week	5th week
Excellent
Good	X	X	X	X	X
Fair
Critical

· *Feces: consistency*
Liquid	X	X	X
Soft				X	X
Normal
Dry

*Feces: odor*
More acrid than normal	X	X	X
Normal
Less acrid than normal				X	X

· *Coat*
Shiny	X	X	X	X	X
Dull
Crinkled

· *Eyes*
Livlier
Normal	X	X	X	X	X
Dull


Weight of subject	26 kg	26 kg	26 kg	26 kg	26 kg

Final comment:

TURVAL B0399 improved the consistency of the feces though it did not resolve the problem of verminosis. After the fifth week the dog was dewormed and the feces returned to normal.