Innovative Probiotic Lactic Yeast for the pharmaceutical, nutritional and agricultural industries

All feeds: TURVAL B0399/500

Probiotic additive for all feeds
with Probiotic Lactic Yeast
( Kluyveromyces BO399 )

Turval B0399/500: informative sheet for technicians

 

STRAIN DEPOSITED IN ACCORDANCE TO THE TREATY OF BUDAPEST and DIRECTIVE 70/524 CEE SHEET N° 2903/00

 

TURVAL B0399

Probiotic additive for all feeds with Probiotic Lactic Yeast

(Kluyveromyces B0399)

INTRODUCTION


Turval B0399 is a new product composed of an exclusive balanced mix of typified and selected lactic yeasts (Kluyveromyces B0399), supported by a wide range of cellular metabolites, fermenting products and hydrolyzed vegetable proteins.The lactic fermenting yeasts (or lactic yeasts) belong to the Kluyveromyces genus and have been used in the food and pharmaceutical industries for quite some time now, giving very interesting results.(Chart 1) Chart 1. List of yeast sources of industrial enzymes (Larpent, 1991) 

Microorganisms
Enzymes
Saccharomyces cerevisiae
Invertasi
Kluyveromyces lactis
Beta-glicosilasi
Saccharomyces alluvius
Amilasi
Saccharomyces diastaticus
Glicoamilasi
Kluyveromyces Fragilis (B0399)
Beta-galattosidasi
Candida cylindraceae
Lipasi
Pichia guillermondii
Alfa-galattosidasi
Cryptococcus albidus
Beta-1,4 xilanoidrolasi

Up until now, the practical use of these yeasts in the animal diet (K. Fragilis or marxianus, in particular) was limited due to the difficulty in stabilizing these microorganisms (and the relative final products) using the standard industrial processes.A lengthy process of research and experimentation resulted in the refinement of a particular biotechnological method to select and typify the lactic yeast and moreover to produce the yeast on the industrial scale which stabilizes the product and exalts the modulation activity of the intestinal flora.The result of this innovative and laborious procedure is the probiotic additive Turval B0399 with lactic yeast Kluyveromyces marxianus/fragilis B0399 .

FUNCTIONAL AND STRUCTURAL CHARACTERISTICS OF  LACTIC YEASTS

Kluyveromyces B0399The principal characteristics of Kluyveromyces fragilis B0399 (which sets it apart from brewer’s yeast, for example) are:· The capacity to grow and develop with extreme facility on a whey medium or other dairy by-products;· The ability to enzymatically degrade lactose (due to the presence of the enzyme beta-galactosidase or lactase in the citosol) in its constituents galactose and glucosesee (fig.1 and chart 2); In regards to the structural components of these active yeasts, it should be noted that the cellular wall contains a considerable quantity of polymers of mannose ( glucomannani, galactomannani e peptidomannani) and of N- acetil-glucosammina organized in complex structures (chitin), as well as the endocellular presence of the natural vitamins C, E and B complex (especially B1 and B2


Fig. 1: Mechanism of action of the enzyme beta-galactosidase

                                       GAL-O-G + H2O = GAL + G

                                 lactose + H2O = galactose + glucose

Chart 2. Glicosidase identified in the yeasts (Bartnett 1981, Suomalainen and Oura 1971) 

Enzima
Yeast
Substratum
Localization
Alfa -amilasi
Candida tropicalis
Amido, destrine
Extracellulare
Treleasi
Saccharomyces cerevisiae
Trealosio
Citosol, m.citoplasmatica
Glicoamilasi
Saccharomyces cerevisiae
Amido, destrine
Extracellulare
Inulinasi
Kluyveromyces fragilis
(B0399)
Inulina
Extracellulare
Maltasi
Saccharomyces cerevisiae
Maltosio, saccarosio
Citosol
Isomaltasi
Saccharomyces cerevisiae
Isomaltosio
Citosol
Invertasi
Saccharomyces cerevisiae
Saccarosio, raffinosio
M. citoplasmatica
Beta-galattosidasi
Lattasi
Kluyveromyces fragilis B0399
Cellobiosio Lattosio
Citosol
Alfa-galattosidasi
Saccharomyces cerevisiae
Raffinosio
M. citoplasmatica

AN INNOVATIVE PRODUCT

Within the ambit of the vast category of probiotic products, Turval B0399 stands out for its unique characteristics, which sets it apart from common brewer’s yeasts and the major part of lactic yeasts. As the in vitro tests conducted at the University of Udine (Italy) have demonstrated, Turval B0399 produces (from the fermentation of the organic substrata) lactic acid and acetic acid much more efficiently than many of the Saccharomyces cerevisiae (Brewer’s yeast) employed in zootechnical diets. (Chart 3) Chart 3. Comparization of fermentation profil of Turval B0399 vs Brewer’s yeast ( pH6,8, 39° C, 24 ore). (Univ. Udine 1999). 

 
TURVAL B0399
Brewer’s yeast
Acido lattico (mg/l)
                2152          
214
Ac. lattico + ac. acetico (mg/l)
3483
330

Compared to other products with a lactobacilli basis, Turval B0399 stands out for its elevated stability at room temperature (at least 24 months) and for the possibility, thanks to its particular resistance, of combining it with antibiotics such as the Ampicillin, Erythromycin, Tetracyclines and Amoxicillin (to which the major part of lactic bacteria is sensitive) and others.

CHARACTERISTICS OF THE SELECTED STRAIN

It is a yeast, Kluyveromyces marxianus (B0399) (Hansen) van der Walt, synonymous of Kluyveromyces fragilis; Genus Kluyveromyces, ascosporigenous, of which morphological characteristics and reproduction system are described. Isolation, purification, preservation, identification and classification were executed according to the dictates of “Bergey’s Manual of Systematic Bacteriology” and according to Lodder e Kreger van Rij, at theUniversitt di Perugia, Italy, Department of Botanical Biology , Applied Microbiology Sector. The mother strain (B0399) is deposited at BCCM – Belgian Coordinated Collection of Microrganism – Colture Collection Mycotheque de l’Université Catholique de Louvain. Biological activity: · Colony Forming Units of Kluyveromyces fragilis (B0399) : >5 x 106CFU/gr · production of b-galactosidase ( lactase )· demolition of lactose in glucose and galactose Morphological characteristics: The morphology of the cell and the mode of reproduction varies depending on whether the strain was cultivated in liquid culture or on “morphology agar”.· Kluyveromyces marxianus ( Hansen ) van der Walt var. Marxianus ( synonymous of Kluyveromyces fragilis ) registration number B0399 at BCCM. · Genus: Kluyveromyces ( Ascosporigenous ).· Vegetative cells: spheroidal, ovoidal, ellipsoidal or cylindrical ( 2,0 - 6,0 ) x ( 3,0 - 10,0 )mm· Reproduction : through single budding ( monopolar ) or in pairs ( bipolar ) and occasionally ( in conditions of anaerobiosis ) in short chains.· Asci : jugated or not jugated, with one or more ascospores ( 4 max.) , evanescent.· Ascospores: crescent-shaped, kidney-shaped, ellipsoidal or spheroidal with a tendency to stick together after liberation.· Culture in agar : colonies of a creamy type, cream colored, with wavy or lobe-shaped border .· Base nitrogenous percentages (mol % G+C): 43.91 ± 0,10 which include values characteristic to the species (see doc 1 attached and also vol 4/5). Physiological characteristics :q vegetative phase : cells predominantly spheroid but also ellipsoidalq reproduction by monopolar budding. q aschi : jugated, of the faded kind, with one or two ascospores up to a maximum of four. q ascospores : kidney-shaped and ellipsoidal with chitinose membrane.

 

- TECHNICAL SCHEET -

 

TURVAL® - B0399/500

Lactic* Yeast® Kluyveromyces marxianus B0399

Complementary Feed -zootechnical use

 

 

Product Description:

 

It is a selected strain of 

Lactic* Yeast Kluyveromyces marxianus fragilis B0399 and the media upon which it was grown.

A high content of protein, amino acids and nucleotides.

 

Analisys:

(%on total product)

(Indicative values)

Ashes 6 - 14

Water content < 9

Crude fibre on dry matter 12 - 15

Proteins 40 - 52

 

 

Ingredienti

Llive Lactic* Yeast Kluyveromyces marxianus fragilis B0399,

(*): No milk ingredients or derivatives included.

 

Process:

Aerobic multistadium process on a sugary substrate and

successive slow and low temperatures spray drying (To>110°C).

Apparence:

beige powder

(Variation in color is common and does not affect product performance.).

 


 

ANALITICAL DATA:

Indicative values.

 

FREE AMINO ACIDS

(after hydrolysis)

 

FREE AMINO ACIDS

(after hydrolysis)

Aspartic Acid

4,5

 

 

 

Glutamic Acid

5,96

 

Leucine

3,84

Alanine

5,22

 

Lysine

3,27

Arginine

1,86

 

Proline

2,2

Phenylalanine

2,09

 

Serine

2,86

Glycine

2,41

 

Tyrosine

1,47

Hydroxyproline

<LQ

 

Threonine

2,78

Isoleucine

2,03

 

Valine

2,47

Histidine

1,08

 

Methionine

0,636

 

 

VITAMINS

 

mg/Kg

Vitamin B1 (thiamin)

12,5

Vitamin B2 (riboflavin)

33,2

Vitamin B5 (pantothenic acid)

33,2

Vitamin B6 (pyridoxine)

13,6

Vitamin B12 (cyanocobalamin)

0,04

Vitamin PP (niacin or nicotinamide)

460

acido folico

2,21

Vitamin E

9,1

Coline clorure

3490

 

 

GENERAL INFORMATIONS

 

 

Expiration date:

 

2 years from production date

Pesticides:

Grain size

EC Regulation 396-2005 and subsequent amendments and additions.

85%< 500 um

Net Weight:

Sack (20 kg each)

Mycotoxins:

EC Regulation 1881-2006and subsequent amendments and additions.

Storage and handling:

Best to store in cooland dry area.

However, storage improves if you keep it at +4-8 ° C.

Foreign material:

absent.

 

OGM FREE – NO ALLERGENS

 

RACCOMANDATIONS

 

Pelletizing

Make pellet at cold temperature (<40°C)

Handling / process indications:

Use all the techniques of good process practice, production and control of the plant.

Adopted mixing procedures must:

-ensure the homogeneity of the final mixture

-performed at room temperature

-does not alter the inherent moisture of the product

 

DIRECTIONS FOR USE

 

For cattle (US-FDA approved)
 
  • Milk cows: 5-8 gr. per head per day;

  • Cattle for fattening: 3-5 gr per head per day

  • Calves: 2-4 gr per head per day

 

We suggest to use the additive Turval B0399 in combination with yeast Saccharomyces cerevisiae: in this case the above dosage can be reduced up to 50%

_______________________________________________________________________

For swine (CE and US-FDA approved)

 

For piglets up to 30kg:

  • Weaning phase and post weaning phase: 0.4-0.5 Kg per 1,000 Kg complete feed

If the piglets were treated since weaning or since the first phase of post-weaning, the suggested inclusion rate can be reduced to: 0.3-0.5 Kg per 1,000 Kg of complete feed

 

In swine over 30 kg: 0.2 Kg per 1,000 Kg of complete feed

 

To obtain best results, Turval B0399 should be administered continually.

_______________________________________________________________________

For horses (US-FDA approved)

 

TURVAL B0399 is added to the daily ration of fodder in the following quantities:

  • Foals: 3-4 gr. per head per day;

  • Weaning colts: 8-12 gr. per head per day;

  • One year old colts, Adults, Broodmares and Stallions: 10-15 gr. per head per day.

 

During the first few days of use and as adjutant, double or triple dosage is recommended.

________________________________________________________________________

For camels and camelids (Llamas and alpacas) (US-FDA approved)

 

TURVAL B0399 is added to the daily ration in the following quantities:

 

  • Crias: 2-4 gr. per head per day

  • Alpacas: 4-6 gr per head per day

  • Llamas: 5-8 gr per head per day

  • Calves: 2-4 gr per head per day

  • Female camels: 5-8 gr gr per head per day

  • Bulls: 8-10 gr per head per day

_______________________________________________________________

For dogs

The additive TURVAL B0399 should be added to daily ration or feed in the following quantities:

  • Small-size dogs (2-10kg) 0.1-0.2 gr per head per day
  • Medium-size dogs (10-20kg) 0.2-0.3 gr per head per day
  • Large-size dogs (20-40kg) 0.3-0.4 gr per head per day

Active, pregnant or nursing dogs: the dosage can be double.

________________________________________________________________________

For broilers

The additive TURVAL B0399 should be added to daily ration or feed in the following percentage:

 

  • Starter-type feed: 300 gr per 100 kg of Starter formulated

  • Grower-type feed: 70 gr per 100 kg of Grower–type feed

  • Finishing-type feed: 20 gr per 100 kg of Finishing-type feed

 

For further information and samples please contact Turval laboratories.


Laboratori Turval Italia Srl – Head Office and Research Dpt: Scientific and Technological Park "L. Danieli"
via Jacopo Linussio, 51 – 33100 Udine (UD) – P. IVA 01883020305